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#Imagej software western blot software#
#Imagej software western blot plus#

Results: The main constituents in TF are AM and robustaflavone. The acute gout mouse model was induced by MSU injection into footpads, and then the paw edema, inflammatory mediators, and histological examination (HE) were analyzed. The inflammatory cytokines and NLRP3 inflammasome activation were determined using enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (RT-PCR), immunofluorescence staining, and western blotting. The cell membrane integrality and morphological characteristics were determined by using Lactate dehydrogenase (LDH) assay kits, propidium iodide (PI) stain, and scanning electron microscopy (SEM). The cellular inflammation model was established by lipopolysaccharide (LPS) or monosodium urate (MSU) stimulation. Methods: LC-MS method was employed to investigate the chemical profile of TF. Purpose: We aimed to investigate the flavonoid extract (TF) and AM's effects on NLRP3 inflammasome in vitro and their preventive effects on gout in vivo. Flavonoids, such as amentoflavone (AM), are the main active components of this medicine. Selaginella moellendorffii has been confirmed effective for the treatment of gout in hospital preparations.

3College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Chinaīackground: Activation of nucleotide oligomerization domain-like receptor protein 3 (NLRP3) inflammasome plays a crucial role in gout.

2Department of Pharmacy, Huanggang Hospital of Traditional Chinese Medicine, Huanggang, China.

1Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription, Key Laboratory of Resources and Chemistry of Chinese Medicine, College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.

Xueyan Zhang 1, Yingbo Liu 1, Guangrui Deng 1,2*, Bisheng Huang 1, Guoyin Kai 3, Keli chen 1 and Juan Li 1*

Cernek J.: Western Blot densitometry analysis - macro tool for ImageJ 1.x.

ImageJ and FiJi authors: for a great Image analysis framework.

(2012 - 2019): bio-scientist and tester in real lab workflow Use the maximum bit depth of your input device. In the other words: Keep the image as it is! Use lossless compression.

Do no apply any image correction, such as as affine transformations (rotation, zoom), change in image pixel levels (contrast, brightness, etc.), or any painting, brushing, wiping, blurring, de-noise, focus, save or re-save in any loss compression.

Preferably acquire all images on the same device.

Prepare all images with the same setting of the digitalization device, gamma, ICC.

#Imagej software western blot software#

Use correct Gamma setting and ICC profile on the digitalization device, image file format and software (ImageJ).

Preferably, use a calibrated digitalization device (scanner, camera) with high DPI (>300).

Evaluate the results in your favourite table / data analysis editor (OpenOffice, R, Excel, SPSS, SAS.

#Imagej software western blot plus#

Plus another file containing all ROIs for later reference with "datetime-ROI.zip" suffix. It will save the results to a file with the same filename as your image with a CSV extension (+ datetime)

Click on the third tool "WB Gel Bands Measure ROIs" to measure and save the results.

The ROI called "background" will be used to subtract the film natural density automatically, so place it wisely.

Select the "Straight line tool" and move all ROIs as needed to avoid debris, scratches and other imperfections that could affect the measurement.

Select the second tool "WB Gel Bands Create ROIs" to create ROIs (rectangles selecting blot bands).

Select the first tool "WB Gel Bands Selection Tool" and draw a line precisely selecting your bands.

Right click on "WB Gel Bands Selection Tool" (the first one on additional palette) and setup WB parameters.Click the icon most to the right in the toolbar ">" and select "WBGelDensitometryTool".for permanent use: copy script file "WBGelDensitometryTool.ijm" into %imagej_root%/macros/toolsets/.for single session use: menu Plugins > Macro > Install.We overwrite files "-ROI.zip" and ".csv".We utilize ImageJ "Overlays" so it will delete all your overlays!.It will not work properly with uneven, warped films / images or images with too small margins.

This tool is not much suitable for other situations.

We expect that blot bands are aligned almost horizontally with almost equal distance between them.Density of image background is measured the same way as bands, on the side of the film, so no rolling-ball, convolution, averages or other techniques are used. It utilizes densitometry measurement of ImageJ and subtracts density of image background. This tool provides a quick and dirty way to measure images of not necessarily straight lines of Western Blot films, dot blots and other silimar bio-scientific images. Western Blot densitometry analysis - macro tool for ImageJ 1.x What is it good for?